Krystof Bankiewicz MD, PhD


Kinetics Foundation Chair in Translational Research and Professor in Residence of Neurological Surgery

Principal Investigator, Brain Tumor Center and the Movement Disorders Research Program

Dr. Bankiewicz's research focus is restorative interventions, specifically drug delivery and gene transfer, in models of Parkinson's disease and brain tumors. His laboratory studies delivery of therapeutic agents into the central nervous system through automated cell-implantation, convection-enhanced and gene transfer-based delivery methods, as well as in vivo applications of MRI and PET to detect in-vivo changes in brain and their correlation with functional outcome.

For the past 12 years, Dr. Bankiewicz has managed his own research group, comprising laboratory technicians, postdoctoral fellows, undergraduate students, and sabbatical visitors. He has trained over 15 postdoctoral fellows and has been involved in many fruitful collaborative projects. His academic teaching has been devoted to training of residents, postdoctoral fellows, and graduate students. He holds several patents, including those for use of neuro-derived fetal cell lines for transplantation therapy, convection-enhanced delivery of adeno-associated virus (AAV) vectors, and convection-enhanced delivery of AAV viral vector with AADC for the treatment of Parkinson's disease.

Read about Dr. Bankiewicz's current research

Education, Training, and Previous Positions

1983: MD, Jagielonian University, Crakow, Poland
1983-1984: Internship in General Surgery, Medical Academy of Warsaw, Poland
1983-1985: Resident, Neuroorthopedic Postgraduate Medical Center, Warsaw, Poland
1983-1985: Assistant Professor, Post-graduate Medical Center, Warsaw, Poland
1985-1988: Visiting Fellow, Surgical Neurology Branch, NINDS, NIH, Bethesda, MD
1988-1991: Visiting Associate Scientist, Surgical Neurology Branch, NINDS, NIH, Bethesda, MD
1991-1992: Visiting Scientist, Surgical Neurology Branch. NINDS, NIH, Bethesda, MD
1991-1993: Chief, Central Nervous System (CNS) Implantation Unit, Surgical Neurology Branch, NINDS, NIH Bethesda, MD
1992-1993: Special Volunteer, Surgical Neurology Branch, NINDS, NIH, Bethesda, MD
1992-1997: Chief, Preclinical Studies, Somatix Therapy Corporation, Alameda, CA
1994-1997: Director, Division of CNS Implantation and Regeneration, The Parkinson's Institute, Sunnyvale, CA
1994-1998: Visiting Scientist, Laboratory for Functional Imaging, Lawrence Berkeley National Laboratory
1996: PhD, Institute of Neurology and Psychiatry, Warsaw, Poland
1997-2001: Acting Chief, Molecular Therapeutics Section, NINDS, NIH, Bethesda, MD

Selected Honors and Awards

1985: Fogarty Fellowship, National Institutes of Health, Bethesda, Maryland
1999: Patent Award, NINDS, NIH, Bethesda, Maryland
2000: Special Act or Service Award, NINDS, NIH, Bethesda, Maryland
2002: Drug Development Prize, Academy of Molecular Imaging
2017: Golden OTIS International Trust Award

Selected Recent Publications

Su X, Fischer DL, Li X, Bankiewicz K, Sortwell CE, Federoff HJ. Alpha-Synuclein mRNA Is Not Increased in Sporadic PD and Alpha-Synuclein Accumulation Does Not Block GDNF Signaling in Parkinson's Disease and Disease Models. Mol Ther. 2017 Oct 4;25(10):2231-2235. doi: 10.1016/j.ymthe.2017.04.018. Epub 2017 May 15. PubMed PMID: 28522034; PubMed Central PMCID: PMC5628770.

Yazdan-Shahmorad A, Tian N, Kharazia V, Samaranch L, Kells A, Bringas J, He J, Bankiewicz K, Sabes PN. Widespread optogenetic expression in macaque cortex obtained with MR-guided, convection enhanced delivery (CED) of AAV vector to the thalamus. J Neurosci Methods. 2018 Jan 1;293:347-358. doi: 10.1016/j.jneumeth.2017.10.009. Epub 2017 Oct 16. PubMed PMID: 29042259.

Louis N, Liu S, He X, Drummond DC, Noble CO, Goldman S, Mueller S, Bankiewicz K, Gupta N, Hashizume R. New therapeutic approaches for brainstem tumors: a comparison of delivery routes using nanoliposomal irinotecan in an animal model. J Neurooncol. 2018 Feb;136(3):475-484. doi: 10.1007/s11060-017-2681-8. Epub 2017 Nov 23. PubMed PMID: 29170909; PubMed Central PMCID: PMC5807192.

Nagahara AH, Wilson BR, Ivasyk I, Kovacs I, Rawalji S, Bringas JR, Pivirotto PJ, Sebastian WS, Samaranch L, Bankiewicz KS, Tuszynski MH. MR-guided delivery of AAV2-BDNF into the entorhinal cortex of non-human primates. Gene Ther. 2018 Apr;25(2):104-114. doi: 10.1038/s41434-018-0010-2. Epub 2018 Mar 13. PubMed PMID: 29535375; PubMed Central PMCID: PMC5924461.

Thomsen GM, Avalos P, Ma AA, Alkaslasi M, Cho N, Wyss L, Vit JP, Godoy M, Suezaki P, Shelest O, Bankiewicz KS, Svendsen CN. Transplantation of Neural Progenitor Cells Expressing Glial Cell Line-Derived Neurotrophic Factor into the Motor Cortex as a Strategy to Treat Amyotrophic Lateral Sclerosis. Stem Cells. 2018 Apr 15. doi: 10.1002/stem.2825. [Epub ahead of print] PubMed PMID: 29656478.

Samaranch L, Blits B, San Sebastian W, Hadaczek P, Bringas J, Sudhakar V, Macayan M, Pivirotto PJ, Petry H, Bankiewicz KS. MR-guided parenchymal delivery of adeno-associated viral vector serotype 5 in non-human primate brain. Gene Ther. 2017 Apr;24(4):253-261. doi: 10.1038/gt.2017.14. Epub 2017 Mar 16. PubMed PMID: 28300083; PubMed Central PMCID: PMC5404203.

Ciesielska A, Samaranch L, San Sebastian W, Dickson DW, Goldman S, Forsayeth J, Bankiewicz KS. Depletion of AADC activity in caudate nucleus and putamen of Parkinson's disease patients; implications for ongoing AAV2-AADC gene therapy trial. PLoS One. 2017 Feb 6;12(2):e0169965. doi: 10.1371/journal.pone.0169965. eCollection 2017. PubMed PMID: 28166239; PubMed Central PMCID: PMC5293261.

Dreisig K, Degn M, Sund L, Hadaczek P, Samaranch L, San Sebastian W, Bankiewicz K, Rahbek Kornum B. Validation of antibodies for neuroanatomical localization of the P2Y(11) receptor in macaque brain. J Chem Neuroanat. 2016 Dec;78:25-33. doi: 10.1016/j.jchemneu.2016.08.002. Epub 2016 Aug 8. PubMed PMID: 27515691.

Hadaczek P, Stanek L, Ciesielska A, Sudhakar V, Samaranch L, Pivirotto P, Bringas J, O'Riordan C, Mastis B, San Sebastian W, Forsayeth J, Cheng SH, Bankiewicz KS, Shihabuddin LS. Widespread AAV1- and AAV2-mediated transgene expression in the nonhuman primate brain: implications for Huntington's disease. Mol Ther Methods Clin Dev. 2016 Jun 29;3:16037. doi: 10.1038/mtm.2016.37. eCollection 2016. PubMed PMID: 27408903; PubMed Central PMCID: PMC4926858.

Spilman PR, Corset V, Gorostiza O, Poksay KS, Galvan V, Zhang J, Rao R, Peters-Libeu C, Vincelette J, McGeehan A, Dvorak-Ewell M, Beyer J, Campagna J, Bankiewicz K, Mehlen P, John V, Bredesen DE. Netrin-1 Interrupts Amyloid-β Amplification, Increases sAβPPα in vitro and in vivo, and Improves Cognition in a Mouse Model of Alzheimer's Disease. J Alzheimers Dis. 2016 Mar 8;52(1):223-42. doi: 10.3233/JAD-151046. PubMed PMID: 27060954.

Han SJ, Bankiewicz K, Butowski NA, Larson PS, Aghi MK. Interventional MRI-guided catheter placement and real time drug delivery to the central nervous system. Expert Rev Neurother. 2016 Jun;16(6):635-9. doi: 10.1080/14737175.2016.1175939. Epub 2016 Apr 20. PubMed PMID: 27054877; PubMed Central PMCID: PMC5553275.

Meneghini V, Lattanzi A, Tiradani L, Bravo G, Morena F, Sanvito F, Calabria A, Bringas J, Fisher-Perkins JM, Dufour JP, Baker KC, Doglioni C, Montini E, Bunnell BA, Bankiewicz K, Martino S, Naldini L, Gritti A. Pervasive supply of therapeutic lysosomal enzymes in the CNS of normal and Krabbe-affected non-human primates by intracerebral lentiviral gene therapy. EMBO Mol Med. 2016 May 2;8(5):489-510. doi: 10.15252/emmm.201505850. Print 2016 May. PubMed PMID: 27025653; PubMed Central PMCID: PMC5128736.

Green F, Samaranch L, Zhang HS, Manning-Bog A, Meyer K, Forsayeth J, Bankiewicz KS. Axonal transport of AAV9 in nonhuman primate brain. Gene Ther. 2016 Jun;23(6):520-6. doi: 10.1038/gt.2016.24. Epub 2016 Mar 8. PubMed PMID: 26953486; PubMed Central PMCID: PMC4893316.

Bankiewicz KS, Sudhakar V, Samaranch L, San Sebastian W, Bringas J, Forsayeth J. AAV viral vector delivery to the brain by shape-conforming MR-guided infusions. J Control Release. 2016 Oct 28;240:434-442. doi: 10.1016/j.jconrel.2016.02.034. Epub 2016 Feb 27. Review. PubMed PMID: 26924352.

Rowland NC, Kalia SK, Kalia LV, Larson PS, Lim DA, Bankiewicz KS. Merging DBS with viral vector or stem cell implantation: "hybrid" stereotactic surgery as an evolution in the surgical treatment of Parkinson's disease. Mol Ther Methods Clin Dev. 2016 Jan 13;3:15051. doi: 10.1038/mtm.2015.51. eCollection 2016. Review. PubMed PMID: 26817024; PubMed Central PMCID: PMC4714520.

Samaranch L, Bringas J, Pivirotto P, Sebastian WS, Forsayeth J, Bankiewicz K. Cerebellomedullary Cistern Delivery for AAV-Based Gene Therapy: A Technical Note for Nonhuman Primates. Hum Gene Ther Methods. 2016 Feb;27(1):13-6. doi: 10.1089/hgtb.2015.129. PubMed PMID: 26757202; PubMed Central PMCID: PMC4761826.

Salegio EA, Bringas J, Bankiewicz KS. MRI-Guided Delivery of Viral Vectors. Methods Mol Biol. 2016;1382:217-30. doi: 10.1007/978-1-4939-3271-9_15. PubMed PMID: 26611589.

Samaranch L, Hadaczek P, Kells AP, Bringas JR, Stockinger D, San Sebastian W, Macayan M, Samineni S, Pivirotto P, Forsayeth J, Bankiewicz KS. Slow AAV2 clearance from the brain of nonhuman primates and anti-capsid immune response. Gene Ther. 2016 Apr;23(4):393-8. doi: 10.1038/gt.2015.87. Epub 2015 Aug 23. PubMed PMID: 26510688.

Seo JW, Ang J, Mahakian LM, Tam S, Fite B, Ingham ES, Beyer J, Forsayeth J, Bankiewicz KS, Xu T, Ferrara KW. Self-assembled 20-nm (64)Cu-micelles enhance accumulation in rat glioblastoma. J Control Release. 2015 Dec 28;220(Pt A):51-60. doi: 10.1016/j.jconrel.2015.09.057. Epub 2015 Oct 5. PubMed PMID: 26437259; PubMed Central PMCID: PMC4688122.

Ciesielska A, Sharma N, Beyer J, Forsayeth J, Bankiewicz K. Carbidopa-based modulation of the functional effect of the AAV2-hAADC gene therapy in 6-OHDA lesioned rats. PLoS One. 2015 Apr 10;10(4):e0122708. doi: 10.1371/journal.pone.0122708. eCollection 2015. PubMed PMID: 25860990; PubMed Central PMCID: PMC4393141.

Forsayeth J, Bankiewicz KS. Transduction of antigen-presenting cells in the brain by AAV9 warrants caution in preclinical studies. Mol Ther. 2015 Apr;23(4):612. doi: 10.1038/mt.2015.35. PubMed PMID: 25849423; PubMed Central PMCID: PMC4395790.

Yang X, Saito R, Nakamura T, Zhang R, Sonoda Y, Kumabe T, Forsayeth J, Bankiewicz K, Tominaga T. Peri-tumoral leakage during intra-tumoral convection-enhanced delivery has implications for efficacy of peri-tumoral infusion before removal of tumor. Drug Deliv. 2016;23(3):781-6. doi: 10.3109/10717544.2014.914987. Epub 2014 May 28. PubMed PMID: 24865286.

Barak S, Wang J, Ahmadiantehrani S, Ben Hamida S, Kells AP, Forsayeth J, Bankiewicz KS, Ron D. Glial cell line-derived neurotrophic factor (GDNF) is an endogenous protector in the mesolimbic system against excessive alcohol consumption and relapse. Addict Biol. 2015 Jul;20(4):629-42. doi: 10.1111/adb.12152. Epub 2014 May 6. PubMed PMID: 24801661.