Krystof Bankiewicz MD, PhD

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Kinetics Foundation Chair in Translational Research and Professor in Residence of Neurological Surgery


Principal Investigator, Brain Tumor Research Center and the Movement Disorders Research Program



Dr. Bankiewicz's research focus is restorative interventions, specifically drug delivery and gene transfer, in models of Parkinson's disease and brain tumors. His laboratory studies delivery of therapeutic agents into the central nervous system through automated cell-implantation, convection-enhanced and gene transfer-based delivery methods, as well as in vivo applications of MRI and PET to detect in-vivo changes in brain and their correlation with functional outcome.

For the past 12 years, Dr. Bankiewicz has managed his own research group, comprising laboratory technicians, postdoctoral fellows, undergraduate students, and sabbatical visitors. He has trained over 15 postdoctoral fellows and has been involved in many fruitful collaborative projects. His academic teaching has been devoted to training of residents, postdoctoral fellows, and graduate students. He holds several patents, including those for use of neuro-derived fetal cell lines for transplantation therapy, convection-enhanced delivery of adeno-associated virus (AAV) vectors, and convection-enhanced delivery of AAV viral vector with AADC for the treatment of Parkinson's disease.

Read about Dr. Bankiewicz's current research


Education, Training, and Previous Positions

1983: MD, Jagielonian University, Crakow, Poland
1983-1984: Internship in General Surgery, Medical Academy of Warsaw, Poland
1983-1985: Resident, Neuroorthopedic Postgraduate Medical Center, Warsaw, Poland
1983-1985: Assistant Professor, Post-graduate Medical Center, Warsaw, Poland
1985-1988: Visiting Fellow, Surgical Neurology Branch, NINDS, NIH, Bethesda, MD
1988-1991: Visiting Associate Scientist, Surgical Neurology Branch, NINDS, NIH, Bethesda, MD
1991-1992: Visiting Scientist, Surgical Neurology Branch. NINDS, NIH, Bethesda, MD
1991-1993: Chief, Central Nervous System (CNS) Implantation Unit, Surgical Neurology Branch, NINDS, NIH Bethesda, MD
1992-1993: Special Volunteer, Surgical Neurology Branch, NINDS, NIH, Bethesda, MD
1992-1997: Chief, Preclinical Studies, Somatix Therapy Corporation, Alameda, CA
1994-1997: Director, Division of CNS Implantation and Regeneration, The Parkinson's Institute, Sunnyvale, CA
1994-1998: Visiting Scientist, Laboratory for Functional Imaging, Lawrence Berkeley National Laboratory
1996: PhD, Institute of Neurology and Psychiatry, Warsaw, Poland
1997-2001: Acting Chief, Molecular Therapeutics Section, NINDS, NIH, Bethesda, MD

Selected Honors and Awards

1985: Fogarty Fellowship, National Institutes of Health, Bethesda, Maryland
1999: Patent Award, NINDS, NIH, Bethesda, Maryland
2000: Special Act or Service Award, NINDS, NIH, Bethesda, Maryland
2002: Drug Development Prize, Academy of Molecular Imaging

Selected Recent Publications

Rosenbluth KH, Martin AJ, Bringas J, Bankiewicz KS. Evaluation of pressure-driven brain infusions in nonhuman primates by intra-operative 7 tesla MRI. J Magn Reson Imaging. 2012 Aug 7. doi: 10.1002/jmri.23771. [Epub ahead of print]

Salegio EA, Samaranch L, Jenkins RW, Clarke CJ, Lamarre C, Beyer J, Kells AP, Bringas J, Sebastian WS, Richardson RM, Rosenbluth KH, Hannun YA, Bankiewicz KS, Forsayeth J. Safety study of adeno-associated virus serotype 2-mediated human Acid sphingomyelinase expression in the nonhuman primate brain. Hum Gene Ther 2012;23(8):891-902.

Mittermeyer G, Christine CW, Rosenbluth KH, Baker SL, Starr P, Larson P, Kaplan PL, Forsayeth J, Aminoff MJ, Bankiewicz KS. Long-term evaluation of a phase 1 study of AADC gene therapy for Parkinson's disease. Hum Gene Ther 2012;23(4):377-81.

Salegio EA, Samaranch L, Kells AP, Mittermeyer G, San Sebastian W, Zhou S, Beyer J, Forsayeth J, Bankiewicz KS. Axonal transport of adeno-associated viral vectors is serotype-dependent. Gene Ther. 2012 Mar 15. doi: 10.1038/gt.2012.27. [Epub ahead of print]

Mendiburu-Eliçabe M, Yin D, Hadaczek P, Zhai Y, Forsayeth J, Bankiewicz KS. Systemic rapamycin alone may not be a treatment option for malignant glioma: evidence from an in vivo study. J Neurooncol 2012;108(1):53-8.

Samaranch L, Salegio EA, San Sebastian W, Kells AP, Foust KD, Bringas JR, Lamarre C, Forsayeth J, Kaspar BK, Bankiewicz KS. Adeno-associated virus serotype 9 transduction in the central nervous system of nonhuman primates. Hum Gene Ther 2012;23(4):382-9.

Hadaczek P, Beyer J, Kells A, Narrow W, Bowers W, Federoff HJ, Forsayeth J, Bankiewicz KS. Evaluation of an AAV2-based rapamycin-regulated glial cell line-derived neurotrophic factor (GDNF) expression vector system. PLoS One 2011;6(11):e27728.

Fiandaca MS, Berger MS, Bankiewicz KS. The use of convection-enhanced delivery with liposomal toxins in neurooncology. Toxins (Basel) 2011;3(4):369-97.

Salegio EA, Samaranch L, Kells AP, Forsayeth J, Bankiewicz K. Guided delivery of adeno-associated viral vectors into the primate brain. Adv Drug Deliv Rev 2012;64(7):598-604.

Kells AP, Forsayeth J, Bankiewicz KS. Glial-derived neurotrophic factor gene transfer for Parkinson's disease: Anterograde distribution of AAV2 vectors in the primate brain. Neurobiol Dis 2012;48(2):228-35.

San Sebastian W, Richardson RM, Kells AP, Lamarre C, Bringas J, Pivirotto P, Salegio EA, Dearmond SJ, Forsayeth J, Bankiewicz KS. Safety and tolerability of magnetic resonance imaging-guided convection-enhanced delivery of AAV2-hAADC with a novel delivery platform in nonhuman primate striatum. Hum Gene Ther 2012;23(2):210-7.

Rosenbluth KH, Eschermann JF, Mittermeyer G, Thomson R, Mittermeyer S, Bankiewicz KS. Analysis of a simulation algorithm for direct brain drug delivery. Neuroimage 2012;59(3):2423-9.

Ziegler RJ, Salegio EA, Dodge JC, Bringas J, Treleaven CM, Bercury SD, Tamsett TJ, Shihabuddin L, Hadaczek P, Fiandaca M, Bankiewicz K, Scheule RK. Distribution of acid sphingomyelinase in rodent and non-human primate brain after intracerebroventricular infusion. Exp Neurol 2011;231(2):261-71.

Forsayeth JR, Bankiewicz KS. AAV9: over the fence and into the woods . . . Mol Ther. 2011 Jun;19(6):1006-7.

Khodr CE, Sapru MK, Pedapati J, Han Y, West NC, Kells AP, Bankiewicz KS, Bohn MC. An alpha-synuclein AAV gene silencing vector ameliorates a behavioral deficit in a rat model of Parkinson's disease, but displays toxicity in dopamine neurons. Brain Res 2011;1395:94-107. doi:10.1016/j.brainres.2011.04.036 


Richardson RM, Kells AP, Martin AJ, Larson PS, Starr PA, Piferi PG, Bates G, Tansey L, Rosenbluth KH, Bringas JR, Berger MS, Bankiewicz KS. Novel Platform for MRI-Guided Convection-Enhanced Delivery of Therapeutics: Preclinical Validation in Nonhuman Primate Brain. Stereotact Funct Neurosurg 2011;89(3):141-151.

Richardson RM, Gimenez F, Salegio EA, Su X, Bringas J, Berger MS, Bankiewicz KS. T2 Imaging in Monitoring of Intraparenchymal Real-time Convection Enhanced Delivery. Neurosurgery 2011;69(1):154-63. doi: 10.1227/NEU.0b013e318217217e

Richardson RM, Kells AP, Rosenbluth KH, Salegio EA, Fiandaca MS, Larson PS, Starr PA, Martin AJ, Lonser RR, Federoff HJ, Forsayeth JR, Bankiewicz KS. Interventional MRI-guided Putaminal Delivery of AAV2-GDNF for a Planned Clinical Trial in Parkinson's Disease Mol Ther 2011;19(6):1048-57. doi:10.1038/mt.2011.11

Rosenbluth KH, Luz M, Mohr E, Mittermeyer S, Bringas J, Bankiewicz KS. Design of an in-dwelling cannula for convection-enhanced delivery. J Neurosci Methods 2011;196(1):118-23.

Fiandaca MS, Salegio EA, Yin D, Richardson RM, Valles FE, Larson PS, Starr PA, Lonser RR, Bankiewicz KS. Human/nonhuman primate AC-PC ratio--considerations for translational brain measurements. J Neurosci Methods 2011;196(1):124-30.

Ciesielska A, Mittermeyer G, Hadaczek P, Kells AP, Forsayeth J, Bankiewicz KS. Anterograde Axonal Transport of AAV2-GDNF in Rat Basal Ganglia. Mol Ther 2011;19(5):922-7.

Laganiere J, Kells AP, Lai JT, Guschin D, Paschon DE, Meng X, Fong LK, Yu Q, Rebar EJ, Gregory PD, Bankiewicz KS, Forsayeth J, Zhang HS. An engineered zinc finger protein activator of the endogenous glial cell line-derived neurotrophic factor gene provides functional neuroprotection in a rat model of Parkinson's disease. J Neurosci 2010;30(49):16469-74.

Fiandaca MS, Bankiewicz KS. Gene therapy for Parkinson's disease: from non-human primates to humans. Curr Opin Mol Ther 2010;12(5):519-29.

Valles F, Fiandaca MS, Eberling JL, Starr PA, Larson PS, Christine CW, Forsayeth J, Richardson RM, Su X, Aminoff MJ, Bankiewicz KS. Qualitative imaging of adeno-associated virus serotype 2-human aromatic L-amino acid decarboxylase gene therapy in a phase I study for the treatment of Parkinson disease. Neurosurgery 2010;67(5):1377-85.

Kells AP, Eberling J, Su X, Pivirotto P, Bringas J, Hadaczek P, Narrow WC, Bowers WJ, Federoff HJ, Forsayeth J, Bankiewicz KS. Regeneration of the MPTP-lesioned dopaminergic system after convection-enhanced delivery of AAV2-GDNF. J Neurosci 2010;30(28):9567-77.

Su X, Kells AP, Aguilar Salegio EA, Richardson RM, Hadaczek P, Beyer J, Bringas J, Pivirotto P, Forsayeth J, Bankiewicz KS. Real-time MR imaging with Gadoteridol predicts distribution of transgenes after convection-enhanced delivery of AAV2 vectors. Mol Ther 2010;18(8):1490-5.

Liu Y, Paliwal S, Bankiewicz KS, Bringas JR, Heart G, Mitragotri S, Prausnitz MR. Ultrasound-Enhanced Drug Transport and Distribution in the Brain. AAPS PharmSciTech. 2010 Jun 8. [Epub ahead of print]

Hadaczek P, Eberling JL, Pivirotto P, Bringas J, Forsayeth J, Bankiewicz KS. Eight years of clinical improvement in MPTP-lesioned primates after gene therapy with AAV2-hAADC. Mol Ther 2010;18(8):1458-61.

Kato T, Natsume A, Toda H, Iwamizu H, Sugita T, Hachisu R, Watanabe R, Yuki K, Motomura K, Bankiewicz K, Wakabayashi T. Efficient delivery of liposome-mediated MGMT-siRNA reinforces the cytotoxity of temozolomide in GBM-initiating cells. Gene Ther. 2010 Jun 3. [Epub ahead of print]

Dickinson PJ, LeCouteur RA, Higgins RJ, Bringas JR, Larson RF, Yamashita Y, Krauze MT, Forsayeth J, Noble CO, Drummond DC, Kirpotin DB, Park JW, Berger MS, Bankiewicz KS. Canine spontaneous glioma: a translational model system for convection-enhanced delivery. Neuro Oncol 2010;12(9):928-40.

Salegio EA, Kells AP, Richardson RM, Hadaczek P, Forsayeth J, Bringas J, Sardi SP, Passini MA, Shihabuddin LS, Cheng SH, Fiandaca MS, Bankiewicz KS. Magnetic resonance imaging-guided delivery of adeno-associated virus type 2 to the primate brain for the treatment of lysosomal storage disorders. Hum Gene Ther 2010;21(9):1093-103.

Yin D, Richardson RM, Fiandaca MS, Bringas J, Forsayeth J, Berger MS, Bankiewicz KS. Cannula placement for effective convection-enhanced delivery in the nonhuman primate thalamus and brainstem: implications for clinical delivery of therapeutics. J Neurosurg 2010;113(2):240-8.

Martínez-Cerdeño V, Noctor SC, Espinosa A, Ariza J, Parker P, Orasji S, Daadi MM, Bankiewicz K, Alvarez-Buylla A, Kriegstein AR. Embryonic MGE precursor cells grafted into adult lls grafted into adult rat striatum integrate and ameliorate motor symptoms in 6-OHDA-lesioned rats. Cell Stem Cell 2010;6(3):238-50.

Hadaczek P, Johnston L, Forsayeth J, Bankiewicz KS. Pharmacokinetics and bioactivity of glial cell line-derived factor (GDNF) and neurturin (NTN) infused into the rat brain. Neuropharmacology 2010;58(7):1114-21.

Gimenez F, Krauze MT, Valles F, Hadaczek PForsayeth J, Bankiewicz KS. Image-guided convection-enhanced delivery of GDNF protein into monkey putamen. Neuroimage. 2010 Jan 18. [Epub ahead of print]

Yin D, Forsayeth J, Bankiewicz KS. Optimized cannula design and placement for convection-enhanced delivery in rat striatum. J Neurosci Methods 2010;187(1):46-51.

Krauze MT, Forsayeth J, Yin D, Bankiewicz KS. Convection-enhanced delivery of liposomes to primate brain. Methods Enzymol 2009;465:349-62.

Christine CW, Starr PA, Larson PS, Eberling JL, Jagust WJ, Hawkins RA, VanBrocklin HF, Wright JF, Bankiewicz KS, Aminoff MJ. Safety and tolerability of putaminal AADC gene therapy for Parkinson disease. Neurology 2009;73(20):1662-9.

Yin D, Valles FE, Fiandaca MS, Bringas J, Gimenez F, Berger MS, Forsayeth J, Bankiewicz KS. Optimal region of the putamen for image-guided convection-enhanced delivery of therapeutics in human and non-human primates. Neuroimage. 2009 Sep 15. [Epub ahead of print]

Valles F, Fiandaca MS, Bringas J, Dickinson P, LeCouteur R, Higgins R, Berger M, Forsayeth J, Bankiewicz KS. Anatomic compression caused by high-volume convection-enhanced delivery to the brain. Neurosurgery 2009;65(3):579-86.

Su X, Kells AP, Huang EJ, Lee HS, Hadaczek P, Beyer J, Bringas J, Pivirotto P, Penticuff J, Eberling J, Federoff HJ, Forsayeth J, Bankiewicz KS. Safety evaluation of AAV2-GDNF gene transfer into the dopaminergic nigrostriatal pathway in aged and parkinsonian rhesus monkeys. Hum Gene Ther 2009;20(12):1627-40.

Varenika V, Kells AP, Valles F, Hadaczek P, Forsayeth J, Bankiewicz KS. Controlled dissemination of AAV vectors in the primate brain [Review]. Prog Brain Res 2009;175:163-72.

Richardson RM, Varenika V, Forsayeth JR, Bankiewicz KS. Future applications: gene therapy [Review]. Neurosurg Clin N Am 2009;20(2):205-10.

Grahn AY, Bankiewicz KS, Dugich-Djordjevic M, Bringas JR, Hadaczek P, Johnson GA, Eastman S, Luz M. Non-PEGylated liposomes for convection-enhanced delivery of topotecan and gadodiamide in malignant glioma: initial experience. J Neurooncol 2009;95(2):185-97.

Yasuhara T, Matsukawa N, Hara K, Maki M, Ali MM, Yu SJ, Bae E, Yu G, Xu L, McGrogan M, Bankiewicz K, Case C, Borlongan CV. Notch-induced rat and human bone marrow stromal cell grafts reduce ischemic cell loss and ameliorate behavioral deficits in chronic stroke animals. Stem Cells Dev 2009;18(10):1501-14.

Hadaczek P, Forsayeth J, Mirek H, Munson K, Bringas J, Pivirotto P, McBride JL, Davidson BL, Bankiewicz KS. Transduction of nonhuman primate brain with adeno-associated virus serotype 1: vector trafficking and immune response. Hum Gene Ther 2009;20(3):225-37.

Eberling JL, Kells AP, Pivirotto P, Beyer J, Bringas J, Federoff HJ, Forsayeth J, Bankiewicz KS. Functional effects of AAV2-GDNF on the dopaminergic nigrostriatal pathway in parkinsonian rhesus monkeys. Hum Gene Ther 2009;20(5):511-8.

Johnston LC, Eberling J, Pivirotto P, Hadaczek P, Federoff HJ, Forsayeth J, Bankiewicz KS. Clinically relevant effects of convection-enhanced delivery of AAV2-GDNF on the dopaminergic nigrostriatal pathway in aged rhesus monkeys. Hum Gene Ther 2009;20(5):497-510.

Kells AP, Hadaczek P, Yin D, Bringas J, Varenika V, Forsayeth J, Bankiewicz KS. Efficient gene therapy-based method for the delivery of therapeutics to primate cortex. Proc Natl Acad Sci U S A 2009;106(7):2407-11.

Fiandaca MS, Varenika V, Eberling J, McKnight T, Bringas J, Pivirotto P, Beyer J, Hadaczek P, Bowers W, Park J, Federoff H, Forsayeth J, Bankiewicz KS. Real-time MR imaging of adeno-associated viral vector delivery to the primate brain. Neuroimage 2009;47 Suppl 2:T27-35.

Fiandaca MS, Forsayeth JR, Dickinson PJ, Bankiewicz KS. Image-guided convection-enhanced delivery platform in the treatment of neurological diseases. Neurotherapeutics 2008;5(1):123-7.
 
Fiandaca M, Forsayeth J, Bankiewicz K. Current status of gene therapy trials for Parkinson's disease. Exp Neurol 2008;209(1):51-57.
 
Krauze MT, Noble CO, Kawaguchi T, Drummond D, Kirpotin DB, Yamashita Y, Kullberg E, Forsayeth J, Park JW, Bankiewicz KS. Convection-enhanced delivery of nanoliposomal CPT-11 (irinotecan) and PEGylated liposomal doxorubicin (Doxil) in rodent intracranial brain tumor xenografts. Neuro Oncol 2007;9(4):393-403.