3D Reconstructions and Renderings
3D Reconstruction of the MRI scans of Gracie, our dog patient, with infusions for brain cancer colored red
MRI 3D reconstruction: cortical (yellow) and brain stem (red) regions were infused with MRI contrast liposomes by CED technique
3D Reconstruction of AAV2-AADC infusions into the putamen: entire MRI cut-away rotated to reveal the putamen (red) within. This and similar work allowed us to optimize AADC gene transfer in PD patients.
3D rendering of our Convection-Enhanced Delivery method: access port attached to skull, cannula aimed at target structure (red), infusion fills target structure (grey), and access port closed. Animation created by John Doval (UCD).
MRI video of real-time infusions of liposomes into a different region of the brain: the brainstem
MRI video of brain tumor therapy (liposomal CPT-11) infused into Gunnar, another dog patient. The tumor is at the lower left.
Example of MRI-guided AAV delivery into the brain: MRI video of AAV2-GFP with the infusion (white) and an overlay of GFP expression (black) showing overlap of delivery and expression
Second MRI video: AAV2-AADC infusion (white) and an overlay of AADC expression (black) showing overlap of delivery and expression
MRI video of brain tumor therapy (liposomal CPT-11) infused into Gracie's tumor.
Post-surgery video of Gracie, running and frolicking a day after receiving CED treatment.
Modeling of convection-enhanced delivery in an agarose gel: standard cannula (left) shows reflux upwards, but our step cannula (right) delivers dye at the cannula tip without reflux.
Example of reflux (right gel) with an unmodified cannula unsuitable for CED. No reflux is observed with our CED cannulae as shown in the middle and left agarose gels.